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Hace 4 días · Polymerase chain reaction (abbreviated PCR) is a laboratory technique for rapidly producing (amplifying) millions to billions of copies of a specific segment of DNA, which can then be studied in greater detail.
Hace 1 día · To assess the expected reduction in immunogenicity more directly, we next performed reverse transcriptase-mediated quantitative polymerase chain reaction (RT-qPCR) to measure the up-regulation of immune response indicators in HEK293T cells .
Hace 1 día · The quantitative polymerase chain reaction (qPCR) is a popular, simple, and rapid method of quantifying nucleic acids. 2 Because it is easy to learn, minimally hazardous, technically simple, and broadly applicable, it is an ideal method for giving hands-on experience to secondary students new to the field.
Hace 2 días · The B chromosome presence was further verified using polymerase chain reaction and FISH (Figs 2c–e, S6,S7). Selected tissues were scored with a marker (Table 1 ; Methods S1 ), and the presence of the B-specific polymerase chain reaction product in individual tissues was assessed.
Hace 2 días · Peptide gene expression levels (Nestin, DCX, Neuritin, MASH1, Neun, BDNF) were analysed by quantitative reverse transcription polymerase chain reaction (RT-PCR). In the OF test, the group–time relationship was found to be statistically different in the parameters of distance travelled and percentage of movement ( p < 0.05).
Hace 4 días · Polymerase chain reaction and immunohistochemistry were also common. Biomechanical testing was the object of only two studies. Conclusions. Although several augmentation strategies have been attempted, none has yielded conclusive results, testifying to a lack of understanding with regard to meniscal healing.
Hace 1 día · To examine whether the depletion of K v 1.3 also influenced infectious virus production, supernatants of reactivated TREx BCBL1-RTA cells were used to reinfect naive cells, and KSHV infection was determined by quantitative reverse transcription polymerase chain reaction (qRT-PCR) or LANA expression.
